Journal: bioRxiv

Article Title: Corilagin controls post-parasiticide schistosome egg-induced liver fibrosis by inhibiting Stat6 signalling pathway

doi: 10.1101/340299

Figure Lengend Snippet: Effect of Corilagin on the expression of ARG1 according to ELISA. Data shown are the mean ± SD from 3 independent experiments. # P< 0.05 vs . normal group; *P< 0.05 VS model group; as determined by Student’s t-test; ## P< 0.01 determined by One-way ANOVA.

Article Snippet: The Mouse ARG1, Fizz1, Ym1, TGFβ and PDGF ELISA kits were purchased from Elabscience Biotechnology Co., Ltd (Wuhan, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Corilagin controls post-parasiticide schistosome egg-induced liver fibrosis by inhibiting Stat6 signalling pathway

doi: 10.1101/340299

Figure Lengend Snippet: Effect of Corilagin on expression of PDGF, Fizz1, Ym1, and TGFβ according to ELISA. Data shown are the mean ± SD from 6 experiment mice. # P< 0.05, ## P< 0.01 vs . normal group; *P< 0.05, ** P< 0.01 VS model group; As determined by Student’s t-test; ### P< 0.01 determined by One-way ANOVA.

Article Snippet: The Mouse ARG1, Fizz1, Ym1, TGFβ and PDGF ELISA kits were purchased from Elabscience Biotechnology Co., Ltd (Wuhan, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Translational Medicine

Article Title: Papillary thyroid cancer organoids harboring BRAF V600E mutation reveal potentially beneficial effects of BRAF inhibitor-based combination therapies

doi: 10.1186/s12967-022-03848-z

Figure Lengend Snippet: List of drugs used in this study

Article Snippet: VEGFR, PDGFRβ , Lenvatinib , MedChemExpress , HY-10981 , 10.

Techniques: DNA Synthesis

Journal: Theranostics

Article Title: Meningeal macrophages regulate fibroblasts to influence meningeal lymphatic function following traumatic brain injury

doi: 10.7150/thno.130263

Figure Lengend Snippet: PDGF-C enhances VEGF-C production by meningeal fibroblasts and promotes the recovery of meningeal lymphatic function after TBI. (A) Schematic of the signaling network through which other cell types regulate meningeal fibroblasts. (B) Dot plot showing macrophage-fibroblast interactions through ligand-receptor pairs, quantified by ligand-receptor scores. (C) Pdgfc/Pdgfra signaling network from other cells to fibroblasts. (D) Violin plots showing the expression levels of Pdgfc across cell types. (E) Violin plots showing the expression levels of Pdgfc in macrophage subsets. (F) Immunofluorescence staining showed colocalization of PDGF-C with macrophages (CD206). (G) ELISA analysis showing PDGF-C levels after TBI and macrophage depletion (n = 5). (H) Experimental design for TBI and uninjured mice with subdural injection of PDGF-C or vehicle. (I) Representative flow cytometry plots and quantification of CD45⁻CD31⁻VEGF-C⁺ cells from TBI mice following subdural injection of PDGF-C or vehicle (n = 5). (J) Immunofluorescence showing mLVs (LYVE-1) and VEGF-C⁺ cells in the meninges of TBI mice after subdural injection of PDGF-C or vehicle and quantification of LYVE-1 coverage, and VEGF-C⁺ cell abundance in these two groups (n = 4). (K) Representative images and quantification of fluorescent beads accumulation in hot spots (n = 4). (L) Representative images and quantification of fluorescent beads accumulation in dCLNs (n = 6 for TBI + Vehicle, n = 5 for TBI + PDGF-C). (M) Representative flow cytometry plots and quantification of the proportion of VEGF-C⁺ cells in NIH/3T3 fibroblasts treated with different concentrations of PDGF-C in vitro (n = 5). (N) Representative images and quantification of VEGF-C⁺ cells in NIH/3T3 fibroblasts treated with different concentrations of PDGF-C in vitro (n = 9). (O) Timeline of experimental design illustrating the viral delivery and TBI induction. (P) Representative immunofluorescence images and quantification showing the number of VEGF-C⁺ cells and LYVE-1 coverage in meninges isolated from TBI mice following AAV9 treatment (n = 4).

Article Snippet: An external injection needle was then repeatedly inserted through the cannula into the subdural space, allowing multiple administrations of PDGF-C (0.1 μg/μL, HY- P73354 , MCE) over several days while minimizing tissue damage from repeated operations.

Techniques: Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Injection, Flow Cytometry, In Vitro, Isolation

Journal: Theranostics

Article Title: Meningeal macrophages regulate fibroblasts to influence meningeal lymphatic function following traumatic brain injury

doi: 10.7150/thno.130263

Figure Lengend Snippet: PDGF - C - mediated recovery of meningeal lymphatic function improves post - TBI neuroinflammation and neurological outcomes . (A) Sensorimotor function assessment in TBI + Vehicle, TBI + PDGF-C, Vehicle, and PDGF-C groups at 3, 5, and 7 days post-TBI using the adhesive removal test, cylinder test, and foot fault test (n = 10 for TBI + Vehicle and TBI + PDGF-C groups; n = 6 for Vehicle and PDGF-C groups). (B) Changes in the proportion and number of infiltrating lymphoid cells within the injured hemisphere (n = 5). (C, D) Representative images and quantification of MBP and SMI32 coverage in the injured hemisphere at 7 days post-TBI (n = 5). (E, F) Representative images and quantification of activated microglia (CD68⁺IBA-1⁺) in the cortex, dentate gyrus of hippocampus and striatum (n = 5). (G, H) Representative images and quantification of GFAP (green) coverage in the injured hemisphere (n = 5). (I) Experimental design for long-term memory and cognitive assessment in TBI + PDGF-C and TBI + Vehicle groups at 28 days post-TBI (n = 10) (J) Representative path tracings during the probe trial, and quantification of swimming speed, escape latency, platform crossings, and time spent in target quadrant in Morris water maze test (n = 10). * TBI + vehicle vs. sham; # TBI + PDGF-C vs. TBI + vehicle. (K, L) Schematic and representative heatmaps during testing, and quantification of time spent in the novel arms in Y-maze test (n = 10). (M) Schematic and quantification of the recognition ratio in the novel object recognition test (n = 10). * P < 0.05, ** P < 0.01, # P < 0.05.

Article Snippet: An external injection needle was then repeatedly inserted through the cannula into the subdural space, allowing multiple administrations of PDGF-C (0.1 μg/μL, HY- P73354 , MCE) over several days while minimizing tissue damage from repeated operations.

Techniques: Adhesive

Journal: Pharmacological research

Article Title: Remote ischemic conditioning attenuates blood-brain barrier disruption after recombinant tissue plasminogen activator treatment via reducing PDGF-CC.

doi: 10.1016/j.phrs.2022.106641

Figure Lengend Snippet: Fig. 4. RIPreC attenuated the phosphorylation of PDGFRα in ischemic brain tissue by reducing the blood levels of PDGF-CC. (A-C) Western blot and quantification of phosphorylated and total PDGFRα in ischemic penumbra cortex at 24 h after intravenous thrombolysis (n = 6 rats per group). Three representative samples were shown. * ** P < 0.001, two-way ANOVA. Data represent mean ± SEM. (D) ELISA analysis of serum PDGF-CC at 24 h after intravenous thrombolysis. (n = 6–9 rats per group). Sham rats received only anesthesia without RIC procedure, while RIPreC rats received both procedures before stroke. * ** P < 0.001, two-way ANOVA. Data represent mean ± SEM. (E) The schematic protocol for healthy rats receiving RIC treatment. Serum samples were collected at day 0 (baseline), day 1, day 7 of RIC, and 24 h after the last RIC procedure (day 8). (F) ELISA analysis of serum PDGF-CC in non-stroke rats after RIC or sham-operation (n = 8 rats per group). Sham rats received only anesthesia without RIC procedure, while RIPreC rats received both procedures. ** P < 0.01, * ** P < 0.001, two-way ANOVA. Data represent mean ± SEM. (G) The schematic protocol for healthy humans receiving 7-day RIC treatment. (H-I) ELISA analysis of serum PDGF-CC levels in 30 healthy humans. One-way ANOVA. Data represent mean ± SEM.

Article Snippet: The rat PDGF-CC protein level was measured using a rat PDGF-CC ELISA kit (CSB-EL017710RA; Cusabio, Houston, TX, USA).

Techniques: Phospho-proteomics, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Pharmacological research

Article Title: Remote ischemic conditioning attenuates blood-brain barrier disruption after recombinant tissue plasminogen activator treatment via reducing PDGF-CC.

doi: 10.1016/j.phrs.2022.106641

Figure Lengend Snippet: Fig. 6. RIC reduces circulating PDGF-CC levels by reducing the PDGF-CC level in the skeletal muscle from RIC-treated hindlimbs and blood platelets. (A-C) Western blot and qRT-PCR analysis of Hif-1α levels in the hindlimbs skeletal muscles receiving RIC or sham procedure. Healthy rats received RIC or sham operation for 7 days and the skeletal muscles from the treated hindlimbs were harvested 24 h after the last round of RIC. Three representative samples were shown (n = 6 rats per group). * P < 0.05, unpaired t-test. Data represent mean ± SEM. (D-F) Western blot analysis of PDGF-CC levels in the skeletal muscles of the hindlimbs receiving RIC or sham procedure (n = 8 rats per group). Three representative samples were shown. Unpaired t-test, * ** P < 0.001. Data represent mean ± SEM. (G-H) ELISA and qRT-PCR analysis of PDGF-CC protein and Pdgfc mRNA levels in the hindlimbs skeletal muscles receiving RIC or sham procedure (n = 8 rats per group) * P < 0.05, * * P < 0.01, unpaired t-test. Data represent mean ± SEM. (I-J) Platelets analysis from blood routine examination in rats received RIC or sham procedure. The blood samples were obtained via inferior vena cava of rats which received a 7-day RIC or sham operation (n = 9 rats per group). Unpaired t-test, * P < 0.05. Data represent mean ± SEM. (K-L) ELISA analysis of PDGF-CC level from platelets or plasma in healthy rats after RIC or sham operation (n = 10 rats per group). Unpaired t-test, * P < 0⋅05. Data represent mean ± SEM. (M) ELISA analysis of PDGF-CC level from indicated tissues harvested from healthy rats after RIC or sham operation (n = 6 rats per group). * ** P < 0.001, Unpaired t-test. Data represent mean ± SEM.

Article Snippet: The rat PDGF-CC protein level was measured using a rat PDGF-CC ELISA kit (CSB-EL017710RA; Cusabio, Houston, TX, USA).

Techniques: Western Blot, Quantitative RT-PCR, Muscles, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Pharmacological research

Article Title: Remote ischemic conditioning attenuates blood-brain barrier disruption after recombinant tissue plasminogen activator treatment via reducing PDGF-CC.

doi: 10.1016/j.phrs.2022.106641

Figure Lengend Snippet: Fig. 7. The protective effect of RIPostC on BBB integrity was also dependent on reduction of the blood PDGF-CC levels. (A) The schematic protocol for RIPostC intervention in rats with embolic stroke. (B-C) Representative images of TTC staining at 72 h after intravenous thrombolysis (n = 8 rats per group). Sham rats received only anesthesia without RIC procedure, while RIPostC rats received both procedures after thrombolysis. * P < 0.05, unpaired t-test. Data represent mean ± SEM. (D) Neurological deficit score at 1 and 3 days after intravenous thrombolysis (n = 10 rats per group). * P < 0.05, two-way ANOVA. Data represent mean ± SEM. (E) qRT-PCR analysis of IL-1β, IL-6, and TNF-α mRNA levels. (n = 8 rats per group). * * P < 0.01, * ** P < 0.001, unpaired t-test. Data represent mean ± SEM. (F) ELISA analysis of serum PDGF-CC (n = 10 rats per group). * * P < 0.01, two-way ANOVA. Data represent mean ± SEM. (G-H) Evans blue leakage at 72 h post intravenous thrombolysis. Recombinant PDGF-CC or normal saline were intravenously injected before and at 24 h and 48 h after thrombolysis (n = 9 rats per group). * P < 0.05, * * P < 0.01, one-way ANOVA. Data represent mean ± SEM. (I-J) The schematic protocol and ELISA analysis for RIPostC intervention in AIS patients with intravenous thrombolysis. Participants received two administrations of RIPostC (22 patients) or sham (20 patients) treatment at 6 h for the first round and 18 h for the second round after intravenous thrombolysis. Unpaired t-test. Data represent mean ± SEM.

Article Snippet: The rat PDGF-CC protein level was measured using a rat PDGF-CC ELISA kit (CSB-EL017710RA; Cusabio, Houston, TX, USA).

Techniques: Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Recombinant, Saline, Injection

Journal: Theranostics

Article Title: Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice.

doi: 10.7150/thno.22144

Figure Lengend Snippet: Figure 1. Harmine increases the number of preosteoclasts and the production of PDGF-BB. (A-C) qRT-PCR analysis of Atp6v0d2, Ctsk and Pdgf-bb expression levels relative to Gapdh in RAW264.7 cells treated with vehicle (DMSO), RANKL and RANKL + harmine. n = 4 per group. (D) Representative images of TRAP staining showing osteoclast and preosteoclast formation from RAW264.7 cells treated with vehicle, RANKL and RANKL + harmine. Scale bar: 50 μm. (E-F) Quantification of TRAP+ osteoclasts (OCs) and preosteoclasts (POCs). n = 3 per group. (G) Detection of PDGF-BB concentration in conditioned media (CM) from RAW264.7 cells treated with vehicle, RANKL and RANKL + harmine by ELISA. n = 5 per group. *P < 0.05 vs. un-induced group (RAW264.7 cells treated with harmine vehicle DMSO), # P < 0.05 vs. RANKL group (RAW264.7 cells treated with RANKL + DMSO).

Article Snippet: The concentrations of PDGF-BB and VEGF in the supernatant of bone marrow or in the CM were measured using a commercial Mouse PDGF-BB ELISA Kit (Cusabio, Wuhan, China) and a Mouse VEGF ELISA Kit (Multi Sciences LTD., Hangzhou, China) according to the protocol provided by the manufacturer.

Techniques: Quantitative RT-PCR, Expressing, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Theranostics

Article Title: Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice.

doi: 10.7150/thno.22144

Figure Lengend Snippet: Figure 6. Harmine emulsion induces type H vessel formation and PDGF-BB production in OVX mice. (A) Representative images of CD31 (red) and Emcn (green) immunostaining in femora from sham, OVX and OVX + harmine mice. BM: bone marrow; GP: growth plate; TB: trabecular bone. Scale bar: 100 µm. (B) Quantification of the ratio of type H vessel (HV; yellow) in different treatment groups. n = 4 per group. TV: total vessels. (C-D) Bone marrow concentrations of PDGF-BB and VEGF from sham, OVX and OVX + harmine mice were detected by ELISA. n = 4 per group. *P < 0.05 vs. sham group, # P < 0.05 vs. OVX group.

Article Snippet: The concentrations of PDGF-BB and VEGF in the supernatant of bone marrow or in the CM were measured using a commercial Mouse PDGF-BB ELISA Kit (Cusabio, Wuhan, China) and a Mouse VEGF ELISA Kit (Multi Sciences LTD., Hangzhou, China) according to the protocol provided by the manufacturer.

Techniques: Emulsion, Immunostaining, Enzyme-linked Immunosorbent Assay